Structural and Molecular Biology

Structural and Molecular Biology
SMB Research Labs

Researchers of the SMB Group develop research in structural biology and glycobiology and have a long-standing interest in a variety of topics that are focused in two main areas: health & disease and environmental impact. The topics are addressed from a functional and structural point of view, relying on complementary expertise in protein production and biochemical, structural and functional characterization (NMR, X-ray crystallography, SAXS, EPR, Moessbauer, Glycoarrays, Kinetics, Microcalorimetry). The optimization and development of new techniques in these fields is a fundamental and active topic of research within the group.

In 2015 a new line of research in functional glycobiology was established focusing on microbial and endogenous recognition systems for development of cancer therapies and to understand the interaction diversity in the human gut microbiome.

Targets of our research are metalloenzymes and we have contributed to the structural and mechanistic elucidation of several enzymes relevant in human health & disease (Aldehyde Oxidases, Peroxidases, etc) as well as on the environment (Formate Dehydrogenase, N2O Reductase) and the results achieved have granted us international recognition.

Other active areas include molecular mechanisms for iron storage, metal tolerance and detoxification of ROS from pathogenic bacteria; mechanisms of protein aggregation in neurodegenerative diseases; bioelectricity production by electroactive bacteria and Extracellular Electron Transfer to allow converting renewable biomass into electricity.

Recent publications
Felix, SS; Laurents, DV; Oroz, J; Cabrita, EJ. 2023. Fused in sarcoma undergoes cold denaturation: Implications for phase separation. PROTEIN SCIENCE, 32, DOI: 10.1002/pro.4521
Pauleta, Sofia R.; Grazina, Raquel; Carepo, Marta S. P.; Moura, José J. G.; Moura, Isabel. 2023. Iron-sulfur clusters – functions of an ancient metal site. Chemistry, Molecular Sciences and Chemical Engineering, DOI: 10.1016/b978-0-12-823144-9.00116-3
Maria Pia Lenza; Leire Egia-Mendikute; Asier Antoñana-Vildosola; Cátia OSoares; Helena Coelho; Francisco Corzana; Alexandre Bosch; Prodhi Manisha; Jon Imanol Quintana; Iker Oyenarte; Luca Unione; María Jesús Moure; Mikel Azkargorta; Unai Atxabal; Klaudia Sobczak; Felix Elortza; James DSutherland; Rosa Barrio; Filipa Marcelo; Jesús Jiménez-Barbero; Asis Palazon; June Ereño-Orbea. 2023. Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b. Nature Communications, DOI: 10.1038/s41467-023-39119-8
Antunes, JMA; Silva, MA; Salgueiro, CA; Morgado, L. 2022. Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens: Biochemical Characterization of Cytochrome CbcL. Frontiers in Microbiology, 13, DOI: 10.3389/fmicb.2022.898015
Karamash, M; Stumpe, M; Dengjel, J; Salgueiro, CA; Giese, B; Fromm, KM. 2022. Reduction Kinetic of Water Soluble Metal Salts by Geobacter sulfurreducens: Fe2+/Hemes Stabilize and Regulate Electron Flux Rates. Frontiers in Microbiology, 13, DOI: 10.3389/fmicb.2022.909109