The X-ray Diffraction facility provides the screening, testing and complete data collection from X-ray diffraction of single crystals, either from protein or small molecule compounds. Data is obtained in the in-house X-ray diffractometer or through access to synchrotron macromolecular crystallography beamlines (ESRF, DIAMOND, SLS, SOLEIL, DESY, ALBA). Diffraction is performed using an X-ray diffractometer (with KAPPA four-circle goniometer), and complete data are collected according to experimental requirements. Dedicated software permits indexing, integration, scaling of data and 3D structure solution. Small-Angle X-ray Scattering (SAXS) experiments can be performed through access to synchrotron facilities. A service for Single Crystal X-ray Structure Determination of small molecules is available (https://www.dq.fct.unl.pt/en/external-services/x-ray-crystallography). The facility integrates the Portuguese Center for Integrated Structural Biology (PCISBIO), an Affiliate Center of Instruct.
Equipment
- X-ray diffractometer with KAPPA four-circle goniometer (Bruker D8 Venture)
- IμS 3.0 microfocus Mo-Kα and Cu-Kα X-ray sources (Bruker)
- Photon 100 CMOS detector
- Cryostream 600 (Oxford CryoSystems)
- ISX Stage for in-situ X-ray crystallography
- Xe chamber for crystal derivatization (Hampton Research)
- Automated nanodrop equipment for protein crystallization (Oryx8, Douglas Instruments)
Responsible
Maria João Romão
PhD in Chemistry, 1989, IST-UL Habilitation in Biochemistry, 2001, FCT NOVA Full Professor, FCT NOVA
Staff
- Ana Luísa Carvalho (Researcher, UCIBIO)
- Teresa Santos-Silva (Assistant Professor, FCT NOVA)
- Clara Gomes (Chemical Crystallography Responsible Scientist, LAQV/UCIBIO)
Funding
- Fundação para a Ciência e Tecnologia
- RECI/BBB-BEP/0124/2012
- COST
- iNEXT
- INSTRUCT-ERIC
