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Add to Calendar 2022-04-28 12:00:00 2022-04-28 13:00:00 GuestSeminars@UCIBIO | Hugh J. Byrne Raman microspectroscopy for cellular and subcellular analysis  Hugh J. Byrne, FOCAS Research Institute, Technological University Dublin, Ireland   Host: Ricardo Franco, UCIBIO NOVA   ZOOM link: https://bit.ly/GuestSeminarsUCIBIO ID da reunião: 865 7786 0516 Senha: 805165   Abstract: In the confocal mode, Raman microspectroscopy can profile the biochemical content of biological cells at a subcellular level, and any changes to it by exogenous agents, such as therapeutic drugs or toxicants. As an exploration of the potential of the technique as a high content, label free analysis technique for cellular and subcellular analysis, this work monitors the spectroscopic signatures associated with the uptake and response pathways of commercial chemotherapeutic agents and polymeric nanoparticles by human lung cells, probing the limitations of the technique in terms of reproducibility and specificity of spectral signatures of subcellular events and pathways. The subtle spectroscopic changes associated with the cellular perturbations are elucidated using a range of multivariate data mining techniques, and the challenge of identifying consistent reproducible label free spectral markers is addressed. It is demonstrated that the signatures are reproducible and characteristic of the cellular event, and can be used, for example, to identify the mode of action of the agent as well as the subsequent cell death pathway, and even mechanisms of cellular resistance. Data mining approaches are discussed and a spectralomics approach is proposed.    Short CV: Professor Hugh J. Byrne is the Head of the FOCAS Research Institute, TU Dublin. He has over 30 years experience in research science and has published over 300 peer reviewed journal and conference papers. He has been responsible for over €25 million in funded projects, including PRTLI Cycles 1,4 and 5 as well as SFI, EI and EU projects and has over 20 years of experience in the management and development of research infrastructure. He has supervised over 45 PhD students to completion. Principal research interests are in applications of spectroscopy and the study of molecular and nano-materials, and biospectroscopy for diagnostics, cytological analysis and nano-bio interactions. Zoom Session UCIBIO info@simbiose.com Europe/Lisbon public
hugh byrne

Raman microspectroscopy for cellular and subcellular analysis 

Hugh J. Byrne, FOCAS Research Institute, Technological University Dublin, Ireland

 

Host: Ricardo Franco, UCIBIO NOVA

 

ZOOM link: https://bit.ly/GuestSeminarsUCIBIO

ID da reunião: 865 7786 0516

Senha: 805165
 

Abstract: In the confocal mode, Raman microspectroscopy can profile the biochemical content of biological cells at a subcellular level, and any changes to it by exogenous agents, such as therapeutic drugs or toxicants. As an exploration of the potential of the technique as a high content, label free analysis technique for cellular and subcellular analysis, this work monitors the spectroscopic signatures associated with the uptake and response pathways of commercial chemotherapeutic agents and polymeric nanoparticles by human lung cells, probing the limitations of the technique in terms of reproducibility and specificity of spectral signatures of subcellular events and pathways. The subtle spectroscopic changes associated with the cellular perturbations are elucidated using a range of multivariate data mining techniques, and the challenge of identifying consistent reproducible label free spectral markers is addressed. It is demonstrated that the signatures are reproducible and characteristic of the cellular event, and can be used, for example, to identify the mode of action of the agent as well as the subsequent cell death pathway, and even mechanisms of cellular resistance. Data mining approaches are discussed and a spectralomics approach is proposed. 

 

Short CV: Professor Hugh J. Byrne is the Head of the FOCAS Research Institute, TU Dublin. He has over 30 years experience in research science and has published over 300 peer reviewed journal and conference papers.
He has been responsible for over €25 million in funded projects, including PRTLI Cycles 1,4 and 5 as well as SFI, EI and EU projects and has over 20 years of experience in the management and development of research infrastructure. He has supervised over 45 PhD students to completion.
Principal research interests are in applications of spectroscopy and the study of molecular and nano-materials, and biospectroscopy for diagnostics, cytological analysis and nano-bio interactions.

GuestSeminars@UCIBIO | Hugh J. Byrne