Primary human hepatocyte spheroids as tools to study the hepatotoxic potential of non-pharmaceutical chemicals
Vânia Vilas Boas, International Iberian Nanotechnology Laboratory - Braga, Portugal
Host: Félix Carvalho, UCIBIO-Porto
ZOOM link: https://bit.ly/GuestSeminarsUCIBIO
ID da reunião: 865 7786 0516
Abstract: Drug-induced liver injury (DILI), including cholestasis, is an important clinical issue and economic burden for pharmaceutical industry and healthcare systems. This is due not only to the delayed manifestation of the injury but also to the poor predictability of DILI in preclinical animal models. There has been a great interest in overcoming these limitations by developing more in vivo-like in vitro models, such as spheroid cultures of primary human hepatocytes (PHH). Taking cholestatic drugs as benchmark, we have used the PHH spheroid model to collect human-relevant in vitro information on the ability of non-pharmaceutical chemicals, such as the biocide paraquat, the food additive tartrazine, and the cosmetic ingredient triclosan, to induce cholestatic hepatotoxicity. This work adds to the applicability of the PHH spheroids to the study of chemicals other than drugs, supporting the model as a reliable and robust tool to depict delayed hepatotoxicity.
Biosketch: My name is Vânia Vilas Boas and I am a Postdoctoral Research Fellow in the newly formed Nanosafety group at the International Iberian Nanotechnology Laboratory (INL), in Braga, Portugal. My current work is dedicated to the development of multiplexed in vitro methodologies for the safety assessment of nanomaterials.
I am a pharmacist (PharmD, 2005), and I hold a Master (2009) and a PhD (2017) in Toxicology from the Faculty of Pharmacy, University of Porto. Prior to joining INL, I was a postdoctoral researcher at the Vrije Universiteit Brussel, funded by a Marie-Curie Individual Fellowship to study the mechanisms of chemical-induced cholestasis. During that period, I had the opportunity to do a long research stay at the Karolinska Institutet, in Stockholm, where I gained skills in 3D cultures of primary human hepatocytes. Since the beginning of my adventure in science, I have explored many different topics within the wider field of Toxicology, such as the analysis of nanomaterials in wastewater samples, the use of magnetic hyperthermia for cancer treatment, and the screening of new antidotes against intoxications with paraquat. My main research interests concern the development of advanced in vitro models and tools for toxicology.